eric,
if you're asking how to get the pc to ack the packet sent by the ecom, you set the appropriate value in the 'flags' (location v+4). by default, a value of 0 says the packet sent by the ecom will require an ack from the pc.
there are always 2 bytes per plc v address.
the byte ordering is dependant on the pc application that's receiving the data, so it's not possible for us to say for sure what byte oredering you need. i can say that normally, to send 192, you'd send in '0x00c0'.
the application value is generated by the sending device and it is unique to each packet sent. when the destination device responds, it uses that application value in the response packet. this is then used to match up the source packet with the response packet. this value is not something you generate, it's done automatically by the ecom.