Make sure no one else is connected to the PLC with another instance of Designer. This can happen with Ethernet connections, or even on the same PC with 2 different Designer sessions with 2 different comm links to the same IP Address, or one link that is USB and one that is Ethernet, for example.
You might try closing (all instanes of) Designer then run Designer and re-opening that project (make note of any message boxes and post them here).